Abstract
Timely and accurate in-season estimation of aboveground biomass (AGB) and yield in winter wheat is crucial for optimizing resources and ensuring food security. Light use efficiency (LUE) models have proven effective in estimating crop gross primary productivity and yield across sites and years due to their strong physiological and ecological mechanisms. However, existing studies are limited to satellite applications and have not utilized unmanned aerial vehicle (UAV) imagery. This study proposed a practical framework for accurate in-season estimation of AGB and yield in winter wheat from UAV imagery by combining a LUE model and machine learning (LUE-ML) across five plot experiments. Subsequently, the scalability of the LUE-ML yield prediction approach was assessed in farmer's fields from five counties of Jiangsu Province, China. The results demonstrated that while the AGB for the heading stage was estimated by combining the retrieved LAI and 20-day accumulated meteorological features, the AGB during the post-heading period could be estimated accurately using the stage-skipping or stage-progressive strategy, with the latter (R (val) (2) = 0.93) outperforming the former (R (val) (2) = 0.84). The combination of one spectral index, LUE-derived AGB, and three 20-day accumulated relative meteorological features (Comb. #6) performed the best (R (cal) (2) = 0.89; R (val) (2) ≥ 0.79) for yield prediction among all combinations. When extended to farmer-field yield prediction across the province, Comb. #6 also achieved acceptable performance. This study suggests the use of LUE-ML models represents a significant step forward towards mechanistic estimation of AGB and yield for cereal crops from UAV imagery.