Abstract
Histamine is among the biogenic amines that are formed during the microbial decarboxylation of amino acids in various food products, posing a significant threat to both food safety and human health. Herein, we present a one-step synthesis of PEGylated gold nanoparticles (PEG-AuNPs) for rapid, simple, and cost-effective colorimetric histamine detection. PEG-AuNPs' surface plasmon resonance (SPR) range at 520-530 nm with a hydrodynamic size distribution of 20-40 nm. Fourier transform infrared (FT-IR) spectra confirmed the reduction of AuNPs at 1645 cm(-1) along with the other observed peaks at 2870, 1350, and 1100 cm(-1) as a strong evidence for the presence of PEG. Upon the addition of histamine to the PEG-AuNP solution, transmission electron microscopy (TEM) highlighted the aggregation of nanoparticles. In addition, red shifting and a decrease in the absorbance of the SPR peak along with the appearance of an additional peak at ∼690 nm was observed in the PEG-AuNP absorption spectra in the presence of histamine. Increasing the PEG concentration in the gold colloids leads to the formation of a protective barrier around the surface of nanoparticles, which influences the colloidal stability by impeding the aggregation of PEG-AuNPs upon histamine addition. The minimum colorimetric response of PEG-AuNPs to histamine concentration is 30 ppm, as assessed by the naked eye. The absorption ratio (A(690)/A(526)) showed a linear dynamic range from 20 to 100 ppm with a limit of detection of 9.357 μM. Additionally, the assay demonstrates a commendable selectivity toward histamine analyte.