Comparison of the host specificities of two bacteroidales quantitative PCR assays used for tracking human fecal contamination
用于追踪人类粪便污染的两种拟杆菌定量 PCR 检测的宿主特异性比较
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作者：Laurie C Van De Werfhorst, Bram Sercu, Patricia A Holden
Abstract
The sewage-associated real-time quantitative PCR (qPCR) assays BacHum and HF183 SYBR were compared for specificity against local fecal sources. Both assays were equally sensitive to sewage, but BacHum showed substantially more false-positive results for cat, dog, gull, and raccoon feces.
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