Abstract
A sensitive electrochemical immunosensor for procymidone detection was developed based on a supramolecular amplification strategy. β-Cyclodextrin (β-CD)-based nanomaterials were employed to immobilize ferrocene derivative (FC)-functionalized antibodies/antigens through host-guest interactions. With the presence of procymidone, the formed β-CD-labeled bioconjugates were immobilized on the antibody-modified electrode after the immunoreaction, indicating fabrication of the immunosensor. The FC/β-CD complexes were with multiplex electroactive species and provided more sites for recognition groups, resulting in signal amplification of the sensor. Monitored with differential pulse voltammetry, the proposed immunosensor exhibited a wide linear range from 5 pM to 0.1 μM with a low detection limit (LOD) of 1.67 pM. The as-prepared immunosensor possessed high sensitivity, specificity, and stability and showed great potential for monitoring procymidone in the field of food safety.