Abstract
Diffusion-weighted imaging (DWI) is a key contrast mechanism in MRI which allows for the assessment of microstructural properties of brain tissues by measuring the displacement of water molecules. Several diffusion models, including the tensor (DTI), kurtosis (DKI), and neurite orientation dispersion and density imaging (NODDI), are commonly used in both research and clinical practice. However, there is currently no standardized method for validating the stability and repeatability of these models over time. This study evaluates the use of a DTI phantom as a standard reference for diffusion MRI model validation. The phantom, along with four healthy volunteers, was scanned repeatedly on different days to assess repeatability and stability. The acquired data were fitted to the diffusion models, with repeatability assessed in the phantom using the coefficient of variation (CoV), while stability in vivo was assessed using the repeatability coefficient (RC). The phantom was consecutively scanned eight times to investigate the impact of gradient coil heating on measurement consistency. Results showed that the phantom provided a highly reproducible reference, with CoVs below 5% across repeated and consecutive acquisitions, confirming the robustness of the diffusion models. In vivo, the low RCs indicated that the models remained stable over time, despite potential physiological variability. This study highlights the essential role of phantoms in diffusion MRI research, providing a reference framework for model validation. Future research will expand on this work to a multi-center study to assess inter-scanner variability, potentially incorporating the phantom into calibration protocols to standardize diffusion MRI measurements across different MRI systems.