Analysis of high-throughput sequencing and annotation strategies for phage genomes

噬菌体基因组高通量测序及注释策略分析

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作者:Matthew R Henn, Matthew B Sullivan, Nicole Stange-Thomann, Marcia S Osburne, Aaron M Berlin, Libusha Kelly, Chandri Yandava, Chinnappa Kodira, Qiandong Zeng, Michael Weiand, Todd Sparrow, Sakina Saif, Georgia Giannoukos, Sarah K Young, Chad Nusbaum, Bruce W Birren, Sallie W Chisholm

Background

Bacterial viruses (phages) play a critical role in shaping microbial populations as they influence both host mortality and horizontal gene transfer. As such, they have a significant impact on local and global ecosystem function and human health. Despite their importance, little is known about the genomic diversity harbored in phages, as

Significance

These DNA preparation, sequencing and annotation strategies enable a high-throughput approach to the burgeoning field of phage genomics.

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