Abstract
Epithelial sodium channels (ENaCs) are of immense importance, controlling Na(+) transport across epithelia and thus playing a central role in all aspects of fluid clearance as well as numerous other functions. Regulation of these channels is critical. Here, we show that haem, a regulator of Na(+) transport, directly influences ENaC activity, decreasing channel-open probability (but not unitary conductance) in inside-out patches (but not outside-out). Conversely, exposure to the protein in the presence of NADPH and at normoxic O(2) tension (requirements for activity of hemeoxygenase) increases channel activity. CO, a product of hemeoxygenase activity, activated ENaC in a manner similar to that of haem plus NADPH. However, under hypoxic conditions, inhibition of ENaC by haem occurred even in the presence of NADPH. These data demonstrate a potent, O(2)-sensitive mechanism for regulation of ENaC, in which hemeoxygenase acts as the O(2) sensor, its substrate and product inhibiting and stimulating (respectively) the activity of ENaC.