Abstract
Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people's health. In the study, MnO&sub2; nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H&sub2;O&sub2;) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO&sub2; with H&sub2;O&sub2;, yellow MnO&sub2; can be converted into colorless Mn2+ to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO&sub2; nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience.