Blastocyst cryopreservation using solid surface vitrification: A preliminary study

利用固体表面玻璃化冷冻保存囊胚:初步研究

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作者:Mohan S Kamath, Ann M Mangalaraj, K Muthukumar, Rosemary Cullinan, Tk Aleyamma, Korula George

Methods

Women undergoing frozen embryo transfer cycles over a 4-year period between 2006 and 2010 were studied. The cryopreservation policy followed was a vitrification protocol performed at the blastocyst stage, using a solid surface (nonimmersion) method. The post-thaw survival rate, implantation rate, clinical pregnancy rate, live birth rate, and neonatal outcome were recorded.

Objective

The objective was to evaluate the effectiveness of a blastocyst cryopreservation program using solid surface vitrification. Setting: This study took place in a university teaching hospital. Study design: Retrospective observational study. Materials and

Results

Eighty-one women underwent 86 frozen embryo transfer cycles. Of the 240 blastocysts warmed, 204 survived giving a cryosurvival rate of 85% (204/240). The clinical pregnancy, implantation, miscarriage, ongoing pregnancy, and live birth rates per transfer were 47%, 29%, 12%, 16%, and 23% respectively. Of the 20 live births, there were 16 singletons and 4 twins. Eleven boys and 13 girls were delivered with no major or minor abnormality detected.

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