Conclusions
Orexin-A elevates the expression of protein markers in human olfactory neuronal progenitor cells to stimulate the differentiation of OSN and enhances the formation of components of the olfactory-specific signaling transduction pathway via orexin receptor type 1.
Methods
The primary olfactory epithelium cells were cultured with or without orexin-A. The neural maturation-related and functional proteins were analyzed through immunofluorescence staining and Western blot. The function of HONCs were evaluated through the synaptic vesicle recycling assay.
Results
The results showed that HONCs in the orexin-A group expressed higher levels of stage-specific markers, including achaete-scute homolog 1, βIII-tubulin, and olfactory marker protein. Additionally, more components of signaling transduction pathways compared to the control group. The orexin-A-mediated differentiation of OSN effect can be nullified with dual orexin receptor antagonist suvorexant and the selective orexin receptor type 1 antagonist SB674042, instead of selective orexin receptor type 2 antagonist TCS-OX2-29. Conclusions: Orexin-A elevates the expression of protein markers in human olfactory neuronal progenitor cells to stimulate the differentiation of OSN and enhances the formation of components of the olfactory-specific signaling transduction pathway via orexin receptor type 1.