日期:
2020 年 — 2026 年
2020
2021
2022
2023
2024
2025
2026
影响因子:

Engineering an Autonucleolytic Mammalian Suspension Host Cell Line to Reduce DNA Impurity Levels in Serum-Free Lentiviral Process Streams

构建自核酸酶哺乳动物悬浮宿主细胞系以降低无血清慢病毒工艺流程中的DNA杂质水平

Howe, Geoffrey; Wasmuth, Matthew; Emanuelle, Pamela; Massaro, Giulia; Rahim, Ahad A; Ali, Sadfer; Rivera, Milena; Ward, John; Keshavarz-Moore, Eli; Mason, Chris; Nesbeth, Darren N

An autonucleolytic suspension HEK293F host cell line for high-titer serum-free AAV5 and AAV9 production with reduced levels of DNA impurity

自核酸溶解悬浮 HEK293F 宿主细胞系,用于生产高滴度无血清 AAV5 和 AAV9,同时降低 DNA 杂质含量

Geoffrey Howe, Mehtap Bal, Matt Wasmuth, Giulia Massaro, Ahad A Rahim, Sadfer Ali, Milena Rivera, Desmond M Schofield, Aminat Omotosho, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N Nesbeth

Serum-free lentiviral vector production is compatible with medium-resident nuclease activity arising from adherent HEK293T host cells engineered with a nuclease-encoding transgene

无血清慢病毒载体生产与利用核酸酶编码转基因工程改造的粘附性 HEK293T 宿主细胞产生的中等驻留核酸酶活性相兼容

Sadfer Ali, Milena Rivera, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N Nesbeth

Minimal impact of ZAP on lentiviral vector production and transduction efficiency

ZAP 对慢病毒载体生产和转导效率的影响极小

Helin Sertkaya, Laura Hidalgo, Mattia Ficarelli, Dorota Kmiec, Adrian W Signell, Sadfer Ali, Hannah Parker, Harry Wilson, Stuart J D Neil, Michael H Malim, Conrad A Vink, Chad M Swanson

Salmonella exploits HLA-B27 and host unfolded protein responses to promote intracellular replication

沙门氏菌利用HLA-B27和宿主未折叠蛋白反应来促进其胞内复制。

Antoniou, Antony Nicodemus; Lenart, Izabela; Kriston-Vizi, Janos; Iwawaki, Takao; Turmaine, Mark; McHugh, Kirsty; Ali, Sadfer; Blake, Neil; Bowness, Paul; Bajaj-Elliott, Mona; Gould, Keith; Nesbeth, Darren; Powis, Simon J