Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (Ab52903)

特异性

This antibody detects Smad3 phosphorylated on Serine 423 and Serine 425. This Smad3 antibody may also detect Smad1, Smad2 and Smad5 phosphorylated at the equivalent sites.

Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, Dot Blot, ICC/IF, IHC-P and WB. Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) was first used in a scientific publication in 2010 and has been cited over 576 times in peer reviewed journals. It's performance in Western Blot in human and mouse samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) has 20 independent reviews from customers. Anti-SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) antibody [EP823Y] (ab52903) specifically detects SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467) (UniProt ID: P84022; Molecular weight: 48kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EP823Y - ab172202. Antibody clone EP823Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, Alexa Fluor ® 594, Alexa Fluor ® 568, Alexa Fluor ® 555, Alexa Fluor ® 750 ( ab310954, ab311071, ab311658, ab312931, ab313143, ab321670 ). Highly cited pan phospho SMAD antibody with >700 citations. Dot blot data to guarantee specificity to SMAD3 (pS423/425) + SMAD5 (pS463/465 ) + SMAD1 (pS463/465) + SMAD2 (pS465/467). Validated for CUT&RUN-seq, a key application to map protein-DNA interactions on a genome-wide scale using NGS. Phosphorylated SMAD proteins, including SMAD3 (pS423/425), SMAD5 (pS463/465), SMAD1 (pS463/465) and SMAD2 (pS465/467), play crucial roles in TGF-beta signaling pathways. These proteins are key regulators of cellular processes such as proliferation, differentiation and apoptosis. Abnormal SMAD signaling is linked to various diseases, including cancer, fibrosis and cardiovascular disorders. Monitoring these phosphorylated SMAD proteins can provide valuable insights into disease mechanisms and therapeutic targets. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies. Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.