BD Pharmingen™ Purified Rat Anti-Mouse Ly-6G (551459)

经验证应用

推荐实验流程

Recommended Assay Procedure: For flow cytometry of leukocyte suspensions, we recommend the use of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142). If Mouse Fc Block™ is used, it is important that the second-step antibody does not react with the 2.4G2 mAb (rat IgG2b, κ); we recommend FITC-conjugated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553896). Other reported applications include immunoprecipitation, immunohistochemical staining (IHC) of paraffin-embedded and acetone fixed frozen sections, and induction of T-cell inhibitory signalling. For IHC, we recommend the use of purified RB6-8C5 mAb in our special formulation for immunohistochemistry, Cat. No. 550291.

制备与储存

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

荧光格式说明

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

产品提示

Since applications vary, each investigator should titrate the reagent to obtain optimal results., Please refer to www.bdbiosciences.com/us/s/resources for technical protocols., Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.