Anti-Vimentin antibody [V9] - Cytoskeleton Marker (Ab8069)

Vimentin, VIM

反应种属:Human, Rat
抗原名称:VIM
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P0.5-5 µg/mL Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
  • WB1 µg/mL
  • ICC/IF0.5-5 µg/mL
  • Flow Cyt (Intra)0.1-1 µg for 10^6 Cells ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Mouse / IgG1
抗体类型Monoclonal
全称Anti-Vimentin antibody [V9] - Cytoskeleton Marker
产品形式Liquid
储存缓冲液pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
储存条件运输条件: Blue Ice 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification

背景信息

What is this antibody validated in? Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Rat samples. What is the molecular weight of Vimentin? Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) specifically detects a band for Vimentin (UniProt: P02543) at a molecular weight of 54kDa. Trusted by the scientific community Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) was first used in a scientific publication in 2002 and has been cited over 170 times in peer-reviewed journals. Reviewed by scientists Anti-Vimentin [V9] - Cytoskeleton Marker (ab8069) has over 25 independent reviews from customers. Specificity confirmed The specificity of Anti-Vimentin antibody [V9] - Cytoskeleton Marker (ab8069) has been confirmed by Immunocytochemistry testing in VIM Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [V9] also available for your convenience: ab8069, Alexa Fluor® 488 - ab195877, Alexa Fluor® 647 - ab195878, HRP - ab196602, Carrier free - ab223871, ab283641, Carrier free - ab302471, ab316575, Carrier free - ab316907 Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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