(10176-2-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, C2C12 cells, mouse brain tissue, rat brain tissue, Jurkat cells, RAW 264, HSC-T6 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比例
- Western Blot (WB)1:2000-1:12000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunofluorescence (IF)/ICC1:200-1:800
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
文献引用 (462)
The engineered probiotic strain Lactococcus lactis MG1363-pMG36e-GLP-1 regulates microglial polarization and gut dysbiosis in a transgenic mouse model of Parkinson's disease.
工程益生菌菌株乳酸乳球菌 MG1363-pMG36e-GLP-1 可调节帕金森病转基因小鼠模型中的小胶质细胞极化和肠道菌群失调
Lysosomal EGFR acts as a Rheb-GEF independent of its kinase activity to activate mTORC1.
溶酶体 EGFR 作为一种 Rheb-GEF，不依赖于其激酶活性，即可激活 mTORC1
Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis.
基于二甲双胍碳点的成骨和蛋白质递送系统，可促进牙周炎中的骨再生
A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer.
全基因组合成致死筛选发现亚精胺合成酶是增强厄达替尼在 FGFR 突变膀胱癌中疗效的靶点
C1q(+) Macrophage-Tumor Cell Interaction Promoted Tumorigenesis via GPR17/PI3K/AKT Pathway Induced DNA Hypermethylation in Nasopharyngeal Carcinoma.
C1q(+)巨噬细胞-肿瘤细胞相互作用通过GPR17/PI3K/AKT通路诱导鼻咽癌DNA高甲基化促进肿瘤发生
An anti-FAP-scFv-functionalized exosome-carrying hydrogel delivers SKI mRNA to fibrotic nucleus pulposus cells to alleviate intervertebral disc degeneration by regulating FOXO3.
一种抗FAP-scFv功能化的外泌体携带水凝胶将SKI mRNA递送至纤维化髓核细胞，通过调节FOXO3来缓解椎间盘退变
Synergistic exacerbation of periprosthetic osteolysis by inflammatory bowel disease and titanium ions: Impaired osteogenesis of BMSCs via PI3K/AKT signaling.
炎症性肠病和钛离子协同加剧假体周围骨溶解：通过 PI3K/AKT 信号通路损害 BMSC 的成骨作用
Matrix stiffness regulates glucose-6-phosphate dehydrogenase expression to mediate sorafenib resistance in hepatocellular carcinoma through the ITGB1-PI3K/AKT pathway.
基质硬度通过 ITGB1-PI3K/AKT 通路调节葡萄糖-6-磷酸脱氢酶的表达，从而介导肝细胞癌的索拉非尼耐药性
Inhibition of serotonin-Htr2b signaling in skeletal muscle mitigates obesity-induced insulin resistance
抑制骨骼肌中血清素-Htr2b信号通路可减轻肥胖引起的胰岛素抵抗。
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