(10366-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HepG2 cells, Jurkat cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue, human kidney tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | HepG2 cells, hiPSC-derived cortical astrocytes (7 DIV) |
| Positive FC (Intra) detected in | HeLa cells, mMSCs cells |
推荐稀释比例
- Western Blot (WB)1:20000-1:100000
- Immunohistochemistry (IHC)1:2500-1:10000
- Immunofluorescence (IF)-P1:50-1:500
- Immunofluorescence (IF)-FRO1:200-1:800
- Immunofluorescence (IF)/ICC1:200-1:800
- Flow Cytometry (FC) (INTRA)0.40 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).
文献引用 (670)
Engineering a halloysite nanotube-enhanced hydrogel 3D skin model for modulated inflammation and accelerated wound healing.
构建埃洛石纳米管增强水凝胶3D皮肤模型，以调节炎症并加速伤口愈合
DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma.
DSS1抑制自噬，从而激活肾细胞癌促转移微环境中的上皮-间质转化
Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring.
确定成纤维细胞衍生的 sFRP2 作为治疗靶点，并设计 siRNA 疗法治疗子宫瘢痕
Phosphorylation of FOXN3 by NEK6 promotes pulmonary fibrosis through Smad signaling
NEK6 磷酸化 FOXN3 通过 Smad 信号促进肺纤维化
BOOST: a robust ten-fold expansion method on hour-scale
BOOST：一种稳健的小时级十倍扩展方法
Hypoxia Induced Lnc191 Upregulation Dictates the Progression of Esophageal Squamous Cell Carcinoma by Activating GRP78/ERK Pathway.
缺氧诱导的 Lnc191 上调通过激活 GRP78/ERK 通路决定食管鳞状细胞癌的进展
Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma.
MYC 过量调控巨噬细胞诱导的染色质重塑，以维持肺腺癌中的微乳头状恶性肿瘤
Deletion of Pyruvate Carboxylase in Tubular Epithelial Cell Promotes Renal Fibrosis by Regulating SQOR/cGAS/STING-Mediated Glycolysis.
肾小管上皮细胞中丙酮酸羧化酶的缺失通过调节 SQOR/cGAS/STING 介导的糖酵解促进肾纤维化
PPDPF preserves integrity of proximal tubule by modulating NMNAT activity in chronic kidney diseases.
PPDPF 通过调节 NMNAT 活性来维持慢性肾脏疾病中近端肾小管的完整性
Application of (68)Ga- and (177)Lu-Labeled FAP Inhibitor in Evaluation and Treatment of Cardiac Fibrosis After Myocardial Infarction.
（68）Ga-和（177）Lu-标记的FAP抑制剂在心肌梗死后心脏纤维化的评估和治疗中的应用
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