(20536-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HEK-293 cells, A549 cells, C6 cells, HeLa cells, mouse colon tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, mouse liver tissue, rat kidney tissue, rat spleen tissue, rat liver tissue |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比例
- Western Blot (WB)1:4000-1:10000
- Immunohistochemistry (IHC)1:50-1:500
- Immunofluorescence (IF)/ICC1:200-1:800
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
文献引用 (1219)
Therapeutic effects of low-intensity transcranial focused ultrasound stimulation on ischemic stroke in rats: An in vivo evaluation using electrical impedance tomography.
低强度经颅聚焦超声刺激对大鼠缺血性中风的治疗效果：采用电阻抗断层扫描的体内评价
Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage.
在蛛网膜下腔出血的小鼠模型中，溴结构域蛋白 4 的敲低会促进神经元铁死亡
Contribution of ferroptosis and SLC7A11 to light-induced photoreceptor degeneration
铁死亡和SLC7A11在光诱导感光细胞退化中的作用
Sequential simulation of regeneration-specific microenvironments using scaffolds loaded with nanoplatelet vesicles enhances bone regeneration.
利用载有纳米片囊泡的支架对再生特异性微环境进行顺序模拟，可增强骨再生
Deletion of Xist repeat B disrupts cell cycle and asymmetric cell division through Usp9x hyperactivation in mice
删除 Xist 重复 B 会通过小鼠中的 Usp9x 过度活化破坏细胞周期和不对称细胞分裂
DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma.
DSS1抑制自噬，从而激活肾细胞癌促转移微环境中的上皮-间质转化
PKA plays a conserved role in regulating gene expression and metabolic adaptation by phosphorylating Rpd3/HDAC1.
PKA 通过磷酸化 Rpd3/HDAC1 在调节基因表达和代谢适应中发挥保守作用
Self-reinforced piezoelectric chip for scaffold-free repair of critical-sized bone defects.
用于无支架修复临界尺寸骨缺损的自增强压电芯片
Single-cell and spatial transcriptome analyses reveal tumor heterogeneity and immune remodeling involved in pituitary neuroendocrine tumor progression.
单细胞和空间转录组分析揭示了垂体神经内分泌肿瘤进展过程中涉及的肿瘤异质性和免疫重塑
F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression.
F-box 蛋白 FBXO32 泛素化并稳定 D 型细胞周期蛋白，从而驱动癌症进展
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