(20874-1-AP)

经验证应用

推荐稀释比例

• Western Blot (WB)1:20000-1:200000
• Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC)1:5000-1:20000
• Immunofluorescence (IF)-P1:200-1:800
• Immunofluorescence (IF)-FRO1:50-1:500
• Immunofluorescence (IF)/ICC1:200-1:800
• Flow Cytometry (FC) (INTRA)0.25 ug per 10^6 cells in a 100 µl suspension

推荐稀释说明

It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also includes N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of epithelial integrity and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cells. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein, and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+. This polyclonal antibody recognizes 120-125 kDa intact E-cadherin and its cleaved fragments of 80-120 kDa.