(19677-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Jurkat cells, HeLa cells, mouse spleen tissue, Staurosporine treated Jurkat cells, rat brain tissue, rat liver tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, mouse brain tissue, mouse eye tissue |
| Positive IF/ICC detected in | NIH/3T3 cells, HeLa cells |
推荐稀释比例
- Western Blot (WB)1:500-1:2000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)1:50-1:500
- Immunofluorescence (IF)-P1:200-1:800
- Immunofluorescence (IF)/ICC1:50-1:500
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa(PMID:9747872). This antibody can recognize p17, p19 and p32 of Caspase 3.
文献引用 (681)
Cytosolic Phospholipase A2 Determines Intercellular Heterogeneity of Stress Granules and Chemotherapy Response
胞质磷脂酶A2决定应激颗粒的细胞间异质性和化疗反应
Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells.
结合多重 CRISPR/Cas9-切口酶和 PARP 抑制剂，可高效、精准地靶向癌细胞
RIPK1 kinase drove brain microvascular endothelial cells death and blood-brain barrier disruption in neonatal Escherichia coli meningitis.
RIPK1 激酶驱动新生儿大肠杆菌脑膜炎中的脑微血管内皮细胞死亡和血脑屏障破坏
Positional BMP signaling orchestrates villus length in the small intestine.
定位性 BMP 信号传导调控小肠绒毛长度
Deacetylation of ANXA2 by SIRT2 desensitizes hepatocellular carcinoma cells to donafenib via promoting protective autophagy.
SIRT2 对 ANXA2 的去乙酰化作用通过促进保护性自噬，使肝细胞癌细胞对多那非尼脱敏
GRP78 Nanobody-Directed Immunotoxin Activates Innate Immunity Through STING Pathway to Synergize Tumor Immunotherapy.
GRP78纳米抗体导向免疫毒素通过STING通路激活先天免疫，从而增强肿瘤免疫疗法的疗效
Microglia-Derived Interleukin-6 Triggers Astrocyte Apoptosis in the Hippocampus and Mediates Depression-Like Behavior.
小胶质细胞衍生的白细胞介素-6 触发海马体中的星形胶质细胞凋亡，并介导抑郁样行为
A Dual Approach with Organoid and CRISPR Screening Reveals ERCC6 as a Determinant of Cisplatin Resistance in Osteosarcoma.
采用类器官和 CRISPR 筛选的双重方法揭示 ERCC6 是骨肉瘤顺铂耐药性的决定因素
PHGDH activation fuels glioblastoma progression and radioresistance via serine synthesis pathway.
PHGDH 激活通过丝氨酸合成途径促进胶质母细胞瘤的进展和放射抗性
Mitochondrial homeostasis restoring peptide-drug conjugates with ROS-responsive NO releasing ability for targeted therapy of myocardial infarction.
具有 ROS 响应性 NO 释放能力的线粒体稳态恢复肽-药物偶联物，用于心肌梗死的靶向治疗
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