用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | recombinant protein, Transfected HEK-293 cells |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:10000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunofluorescence (IF)/ICC1:200-1:800
- Flow Cytometry (FC) (INTRA)0.25 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.
文献引用 (549)
Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation.
丝氨酸/苏氨酸/酪氨酸激酶 1 通过 GSK3β 隔离介导的 Wnt/β-catenin 通路过度激活驱动胰腺癌的发生
YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies
YTHDF2促进B细胞恶性肿瘤中的ATP合成和免疫逃逸。
NAP1L1 degradation by FBXW7 reduces the deubiquitination of HDGF-p62 signaling to stimulate autophagy and induce primary cisplatin chemosensitivity in nasopharyngeal carcinoma.
FBXW7 介导的 NAP1L1 降解减少了 HDGF-p62 信号的去泛素化，从而刺激自噬并诱导鼻咽癌对顺铂化疗的敏感性
Epigenetic silencing of interleukin-10 by host-derived oxidized phospholipids supports a lethal inflammatory response to infections
宿主来源的氧化磷脂通过表观遗传机制沉默白细胞介素-10，从而促进对感染的致命性炎症反应。
Demyelination-derived lysophosphatidylserine promotes microglial dysfunction and neuropathology in a mouse model of Alzheimer's disease
脱髓鞘衍生的溶血磷脂酰丝氨酸促进阿尔茨海默病小鼠模型中的小胶质细胞功能障碍和神经病理学改变
Temporal control of progenitor competence shapes maturation in GABAergic neuron development in mice.
祖细胞能力的时序控制塑造了小鼠 GABA 能神经元发育的成熟过程
In vivo measurement of an Apelin gradient with a genetically encoded APLNR conformation biosensor.
利用基因编码的 APLNR 构象生物传感器对 Apelin 梯度进行体内测量
Axin formation inhibitor 1 aggravates hepatic ischemiaâreperfusion injury by promoting the ubiquitination and degradation of PPARβ.
Axin 形成抑制剂 1 通过促进 PPARβ 的泛素化和降解来加重肝脏缺血再灌注损伤
DSS1 inhibits autophagy to activate epithelial-mesenchymal transition in a pro-metastatic niche of renal cell carcinoma.
DSS1抑制自噬，从而激活肾细胞癌促转移微环境中的上皮-间质转化
Amylopectin branch trimming and biosynthesis elucidated by the rice isoamylase ISA1-ISA2 heterocomplex.
水稻异淀粉酶 ISA1-ISA2 异源复合物阐明了支链淀粉的分支修剪和生物合成
- 1
- 2
- 3
- 4
- 5
- 6
- 55