(60008-1-Ig)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | multi-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue |
| Positive IHC detected in | mouse heart tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:50000
- Immunohistochemistry (IHC)1:50-1:500
- Immunofluorescence (IF)-P1:500-1:2000
- Immunofluorescence (IF)/ICC1:400-1:1600
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
文献引用 (796)
Comprehensive profiling of lipid metabolic reprogramming expands precision medicine for HCC.
对脂质代谢重编程进行全面分析，可扩大肝细胞癌精准医疗的应用
USP7 V517F mutation as a mechanism of inhibitor resistance.
USP7 V517F 突变是抑制剂耐药性的机制
FoxO3 controls cardiomyocyte proliferation and heart regeneration by regulating Sfrp2 expression in postnatal mice.
FoxO3 通过调节出生后小鼠的 Sfrp2 表达来控制心肌细胞增殖和心脏再生
ATG2A acts as a tether to regulate autophagosome-lysosome fusion in neural cells.
ATG2A 作为一种连接蛋白，调节神经细胞中的自噬体-溶酶体融合
Elevated Histone Lactylation Mediates Ferroptosis Resistance in Endometriosis Through the METTL3-Regulated HIF1A/HMOX1 Signaling Pathway.
组蛋白乳化水平升高通过 METTL3 调控的 HIF1A/HMOX1 信号通路介导子宫内膜异位症中的铁死亡抵抗
HUWE1-Mediated Degradation of MUTYH Facilitates DNA Damage and Mitochondrial Dysfunction to Promote Acute Kidney Injury.
HUWE1 介导的 MUTYH 降解促进 DNA 损伤和线粒体功能障碍，从而导致急性肾损伤
Carnosic Acid Directly Targets STING C-Terminal Tail to Improve STING-Mediated Inflammatory Diseases.
鼠尾草酸直接靶向 STING C 端尾部，改善 STING 介导的炎症性疾病
Hyperinsulinemia-induced upregulation of adipocyte TPH2 contributes to peripheral serotonin production, metabolic dysfunction, and obesity.
高胰岛素血症诱导的脂肪细胞 TPH2 上调导致外周血清素生成、代谢功能障碍和肥胖
RNF138 regulates skeletal muscle differentiation via the Wnt/β-catenin signaling pathway.
RNF138 通过 Wnt/β-catenin 信号通路调节骨骼肌分化
IL-21 Loading CaMnCO3 Vitality Backpacks Boost CAR-T Cell Synergistic Immunotherapy
IL-21负载CaMnCO3活力背包增强CAR-T细胞协同免疫疗法
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