(60004-1-Ig)

经验证应用

推荐稀释比例

• Western Blot (WB)1:50000-1:500000
• Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC)1:3000-1:16000
• Immunofluorescence (IF)/ICC1:400-1:1600
• Flow Cytometry (FC) (INTRA)0.40 ug per 10^6 cells in a 100 µl suspension

推荐稀释说明

It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.

What is the molecular weight of GAPDH? The molecular weight of GAPDH is 36 kD. What is GAPDH? GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic production of ATP and pyruvate (PMID: 21895736). What is the function of GAPDH? GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 11035021). Where in the cell is GAPDH localized? GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus (PMIDs: 21895736, 12829261). Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear functions? Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is useful as a control for Western blots and RT-PCR. What are the known protein-protein interactions of GAPDH? GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407). Note For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.