用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, 3T3-L1 cells, 4T1 cells, RAW 264.7 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue, human liver cancer tissue, human placenta tissue, human tonsillitis tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, mouse heart tissue |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
文献引用 (73)
PRMT1-methylated MSX1 phase separates to control palate development
PRMT1 甲基化的 MSX1 相分离以控制腭发育
PU.1/Spi1 exacerbates ischemia-reperfusion induced acute kidney injury via upregulating Gata2 and promoting fibroblast activation.
PU.1/Spi1 通过上调 Gata2 和促进成纤维细胞活化，加剧缺血再灌注引起的急性肾损伤
PKM2-mediated collagen XVII expression is critical for wound repair
PKM2介导的胶原蛋白XVII表达对伤口修复至关重要
Curcumin attenuates myocardial ischemia‑reperfusion‑induced autophagy‑dependent ferroptosis via Sirt1/AKT/FoxO3a signaling
姜黄素通过 Sirt1/AKT/FoxO3a 信号减弱心肌缺血再灌注诱导的自噬依赖性铁死亡
Spautin-1 inhibits the growth of diffuse large B-cell lymphoma by inducing mitochondrial damage-mediated PANoptosis and anti-tumor immunity.
Spautin-1 通过诱导线粒体损伤介导的 PANoptosis 和抗肿瘤免疫来抑制弥漫性大 B 细胞淋巴瘤的生长
The Effect of Adipose-Derived Stem Cell (ADSC)-Exos on the Healing of Autologous Skin Grafts in Miniature Pigs.
脂肪来源干细胞（ADSC）-外泌体对小型猪自体皮肤移植愈合的影响
FGA, a new target of histone acetylation, inhibits apoptosis of granulosa cells in follicles.
FGA 是组蛋白乙酰化的新靶点，可抑制卵泡中颗粒细胞的凋亡
Mitochondrial Calcium Uniporter-Mediated Regulation of the SIRT3/GSK3β/β-Catenin Signaling Pathway in Vascular Remodeling.
线粒体钙单向转运蛋白介导的 SIRT3/GSK3β/β-catenin 信号通路在血管重塑中的调控
Triple‑negative breast cancer cell‑derived piR‑31115 promotes the proliferation and migration of endothelial cells via METTL3‑mediated m6A modification of YAP1
三阴性乳腺癌细胞来源的piR-31115通过METTL3介导的YAP1 m6A修饰促进内皮细胞的增殖和迁移
Identification of the clinical value and biological effects of TTN mutation in liver cancer.
确定 TTN 突变在肝癌中的临床价值和生物学效应
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