Anti-TSG101 antibody [EPR7130(B)] (Ab125011)

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

反应种属:Human, Mouse, Rat
抗原名称:Tumor susceptibility gene 101 protein
应用:WB, IHC, FCM, IF/ICC

用户验证的应用

推荐稀释比例

  • IHC-P1/300 For unpurified use at 1/30. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • WB1/1000 - 1/10000
  • ICC/IF1 µg/mL For unpurified use at 1/10. This product gave a positive signal in HeLa fixed with 100% methanol (5 min).
  • Flow Cyt (Intra)1/20 For unpurified use at 1/3. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

详细信息

免疫原The exact immunogen used to generate this antibody is proprietary information.
宿主/亚型Rabbit / IgG
抗体类型Monoclonal
全称Anti-TSG101 antibody [EPR7130(B)]
产品形式Liquid
储存缓冲液pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
储存条件运输条件: Conditional Ambient 推荐的短期储存时间: 1-2 weeks 推荐的短期储存条件: +4°C 推荐的长期储存条件: -20°C 储存信息: Avoid freeze / thaw cycle
纯化方式Affinity purification Protein A

背景信息

Anti-TSG101 antibody [EPR7130(B)] (ab125011) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB. Anti-TSG101 antibody [EPR7130(B)] (ab125011) was first used in a scientific publication in 2014 and has been cited over 603 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-TSG101 antibody [EPR7130(B)] (ab125011) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-TSG101 antibody [EPR7130(B)] (ab125011) has 10 independent reviews from customers. Anti-TSG101 antibody [EPR7130(B)] (ab125011) specifically detects TSG101 (UniProt ID: Q99816; Molecular weight: 44kDa) and is sold in a convenient trial size to enable initial testing (10 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EPR7130(B) - ab207567. Antibody clone EPR7130(B) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 488, Alexa Fluor ® 647, PE, Alexa Fluor ® 555, APC ( ab207663, ab207664, ab209927, ab214404, ab316299 ). Tumor Susceptibility Gene 101 (TSG101) is involved in the endosomal sorting complex required for transport (ESCRT) pathway, which is crucial for cellular processes like vesicle trafficking and degradation. Alterations in TSG101 expression are linked to various cancers, including breast cancer and glioblastoma, where it can act as both a tumor suppressor and an oncoprotein depending on the context. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies.

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