Anti-M13 Antibody, Mouse Monoclonal (11973-MM05T)

经验证应用

推荐稀释说明

Optimal concentrations/dilutions should be determined by the end user.

特异性

The antibody binds specifically to phage coat proteins of fd phage or M13 phage

制备方法

This product is a recombinant monoclonal antibody expressed from HEK293 cells.

运输条件

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form. References Messing, J. et al., 1993, Methods Mol. Biol. 23: 9-22. Mori, K. et al., 1996, Antiviral Res. 31 (1-2): 79-86. Sidhu, S.S. et al., 2001, Biomol Eng. 18 (2): 57-63. Sitohy, M. et al., 2006, J Agric Food Chem. 54 (11): 3800-6. Khalil, A.S. et al., 2007, Proc Natl Acad Sci. USA. 104 (12): 4892-7.