Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.
Mitochondrial Isolation and Real-Time Monitoring of MOMP.
线粒体分离及MOMP实时监测
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作者:Luna-Vargas Mark P A, Mohammed Jarvier N, Gelles Jesse D, Chipuk Jerry Edward
| 期刊: | Methods in Molecular Biology | 影响因子: | 0.000 |
| 时间: | 2019 | 起止号: | 2019;1877:121-130 |
| doi: | 10.1007/978-1-4939-8861-7_8 | 研究方向: | 其它 |
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