GATA3 regulates Th1/Th2 balance in allergic rhinitis by interacting with RUNX1.

OBJECTIVE: To investigate the action mechanism of GATA3 and Runt-related transcription factor 1(RUNX1) in modulating regulatory the Th1/Th2 cell differentiation and treat allergic rhinitis (AR). METHODS: Established AR model mice by ovalbumin (OVA) and isolated the peripheral blood monocytes (PBMCs), evaluating behavioral changes of AR mice. Analysis of histopathological characteristics by HE. sIgE, IL-4, and IFN-γ were detected by ELISA. RT-qPCR and Western blot detected the expression of GATA3, T-bet, and RUNX1. Using immunofluorescence to detect the expression of GATA and T-bet. RESULTS: Knockout GATA3 suppressed the expression of GATA3 and concentration of sIgE and IL-4, and promoted the expression of T-bet and concentration of IFN-γ. Knockout GATA3 alleviates inflammation in AR mice by increasing the CD4(+) IFN-γ(+) cell rate and decreasing the CD4(+) IL-4(+) cell rate. RUNX1 and GATA3 were both expressed in the nucleus, GATA3 could bind and interact with RUNX1, si-RUNX1 reversed the effect of sh-GATA3, RUNX1 regulated the balance of Th1/Th2 to alleviate inflammation in AR mice by down-regulating expression of GATA3. CONCLUSION: GATA3 regulated the balance of Th1/Th2 cells maybe by the interaction between RUNX1 to improve anti-allergic and anti-inflammatory potential strategies in AR therapy.