Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories.
Single cell transcriptomics of neighboring hyphae of Aspergillus niger.
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作者：de Bekker Charissa, Bruning Oskar, Jonker Martijs J, Breit Timo M, Wösten Han A B
| 期刊： | Genome Biology | 影响因子： | 9.400 |
| 时间： | 2011 | 起止号： | 2011 Aug 4; 12(8):R71 |
| doi： | 10.1186/gb-2011-12-8-r71 | ||
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