Abstract
Trichomonascaceae is the largest family of ascomycetous yeast in the order Saccharomycetales. In spite of the extensive body of research on Trichomonascaceae in China, there remain new species to be discovered. Here, we describe four new species isolated from several rotting wood samples from Henan Province, Central China. Phylogenetic analysis of a combined ITS and nrLSU dataset with morphological studies revealed four new species in the Trichomonascaceae: Diddensiellaluoyangensis, Sugiyamaellacylindrica, Su.robnettiae, and Zygoascusdetingensis. Clustering in the Diddensiella clade, D.luoyangensis' closest neighbour was D.transvaalensis. Meanwhile, Su.cylindrica clustered in the Sugiyamaella clade closest to Su.marilandica and Su.qingdaonensis. Also clustering in the Sugiyamaella clade, Su.robnettiae was most closely related to Su.chuxiongensis. Finally, Z.detingensis occupied a distinct and separated basal branch from the other species of the genus Zygoascus. These results indicate a high species diversity of Trichomonascaceae.