Abstract
During a survey of microfungal diversity in Guizhou Province, southwestern China, four helicosporous taxa were collected and isolated from decaying wood in both freshwater and terrestrial habitats. Phylogenetic analyses based on LSU, ITS, tef1-α, and rpb2 sequences, along with morphological assessments, revealed two new species: Neohelicomyces saprobicus and N. uniramulosus. Morphologically, Neohelicomyces saprobicus differs from its close relative N. sexualis by its asexual mode of reproduction, in contrast to the sexual reproduction observed in N. sexualis. Neohelicomyces uniramulosus is distinguished from its close relative N. helicosporus and N. hyalosporus by its conidiophores. Detailed morphological descriptions, illustrations, and taxonomic notes are provided. In addition, the present study summarizes the main morphological characteristics, host associations, and geographical distributions of Neohelicomyces.