Abstract
In this study, three new basidiomycetous yeast species of the genus Vishniacozyma are proposed for strains isolated from soil, Scolytus scolytus frass, fruits and kombucha tea. A complex analysis of the new isolates together with the described species by genetic, phylogenetic, MALDI-TOF MS profiling and phenotypic characterization revealed significant differences, allowing us to propose three new species: V. pseudofoliicola sp. nov. (holotype KBP Y-7396T, MycoBank no.: MB861627), V. kombuchae sp. nov. (holotype KBP Y-7350T, MycoBank no.: MB861628) and V. fructicola sp. nov. (holotype KBP Y-6599T, MycoBank no.: MB861629). These descriptions contribute to the expansion of knowledge regarding species diversity of the genus Vishniacozyma.