Abstract
This study explores the microbial-assisted synthesis of nanoparticles from chalcopyrite ore and its tailings, emphasizing their potential applications in sustainable agriculture. Addressing the gap between research and practical field applications, the study employs microbial diversity to recover valuable elements as nanoparticles through green methods. Organic acids and metabolites produced by microorganisms facilitated the breakdown of mineral ores into nanoparticles. Optimal conditions (28 °C, pH 3.8) yielded nanoparticles (15-200 nm) after 72 h, as analysed by Dynamic Light Scattering and Scanning Electron Microscopy. Glycine encapsulation was confirmed via UV spectroscopy. In vitro experiments on Cicer arietinum (L.) demonstrated enhanced plant growth, including increased seed germination, branching, and early flowering, highlighting the potential of these nanoparticles in agricultural enhancement.