Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay
使用定量双重时间分辨荧光免疫分析法测量实验性诺沃克病毒感染的血清学反应
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作者：Owen Kavanagh, Mary K Estes, Amanda Reeck, Ravikiran M Raju, Antone R Opekun, Mark A Gilger, David Y Graham, Robert L Atmar
Abstract
A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).
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