Abstract
Aflatoxins contamination of maize exhibits a serious threat to human and animal health over the past few decades. To protect the safety of food commodities, regular monitoring for afltoxins in food is necessary. In the proposed study, we have followed a rapid and sensitive biosensor approach as well as thin layer chromatography method for quantification of aflatoxins. Our data demonstrate that all the samples tested were beyond the safety level of aflatoxins as determined by Food and Drug Administration and European Union. Results of fungal mycoflora evidenced the massive presence of Aspergillus species (75 %) followed by Fusarium (11 %), Penicillium (8 %) and Trichoderma (6 %) as characterized by biochemical and sporulation properties. Use of internationally developed biosensor for detection of fungal toxin in this work is the first approach that was utilized in the developing country like Ethiopia. In the end, we conclude that fungal contaminant and there metabolites are potential threat to the agricultural industry and require urgent intervention.