Abstract
The petroleum hydrocarbons in seawater have been worldwide concern contaminants. Biological method, with the advantages of low cost, minimal environmental impact, and no secondary pollution, is a promising method for petroleum hydrocarbon treatment. In this study, two strains, identified as Stenotrophomonas acidaminiphila and Ochrobactrum, were demonstrated to possess the ability to degrade petroleum hydrocarbons. The mixed culture composed of Stenotrophomonas acidaminiphila and Ochrobactrum at a 2:1 ratio was able to achieve 79.41% degradation of the total petroleum hydrocarbons after 5 days. Besides, the average removal efficiencies of C10-C30 components in petroleum hydrocarbons by Stenotrophomonas acidaminiphila, Ochrobactrum, and mixed culture were 62.98%, 59.14% and 73.30%, respectively. The possible degradation pathways of petroleum hydrocarbons had been speculated through gas chromatography-mass spectrometry (GC-MS) and differential gene expression metabolomics analyses. The toxicity of products from the biodegradation of petroleum hydrocarbons was greatly reduced.