Abstract
Soil microbial communities face the combined pressures of climate change and biodiversity loss, yet how these stressors interact to shape ecosystem function remains a critical uncertainty. To investigate this, we established a constructed grassland plant community and conducted a fully factorial experiment manipulating plant diversity (1, 3, and 6 species), temperature (ambient, +2 °C), and precipitation (ambient, +50%). High-throughput 16S rRNA gene sequencing revealed that plant diversity exerted a stronger influence on soil bacterial community structure than did warming or precipitation changes. Beta diversity analysis revealed a distinct clustering of bacterial communities corresponding to the plant diversity gradient. This shift was characterized by a consistent enrichment of the metabolically versatile genus Sphingomonas in medium-diversity plots that experienced elevated precipitation, suggesting a predicted potential for enhanced organic matter decomposition. Despite overall stability in alpha diversity, the interaction between plant diversity and warming significantly modulated bacterial diversity and dominance patterns. Our findings highlight that plant diversity plays a key role in mediating soil bacterial responses to simulated climate factors in the short term. Incorporating these plant-soil feedback mechanisms into ecological models appears crucial for advancing predictions of ecosystem dynamics under future climate conditions.