Abstract
This study explored the synthesis and characterization of pectin-based composites containing encapsulated propolis and sea buckthorn oil. Both propolis and sea buckthorn oil are well known for their antioxidant and antimicrobial properties. To mitigate their sensitivity to environmental degradation, these compounds were encapsulated within a pectin matrix. The composites were prepared using an emulsification technique and subsequently for their physicochemical properties via scanning electron microscopy (SEM), ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC), as well as color and mechanical testing. The results showed that freeze-dried samples exhibited heterogeneous, bubble-like structures containing nanocapsules (800-2000 nm), whereas for the film samples, the capsules were visibly embedded within the matrix. The study shows that this three-component system exhibits synergistic potential. Encapsulation significantly improved the UV barrier properties and the antioxidant activity of the nanocomposites, which demonstrated greater antioxidant capacity. Microbiological assays revealed that the pectin-based composites containing encapsulated propolis and sea buckthorn oil exhibited strong antibacterial activity, particularly against Gram-positive bacteria such as Streptococcus and Staphylococcus spp. The composites also demonstrated hydrophobic surface characteristics and reduced crystallinity, which correlates with their potential for controlled release. These results underscore the applicability of pectin-propolis-sea buckthorn oil composites as effective natural preservatives or functional ingredients in food systems, due to their high antioxidant and antimicrobial efficacy.