Effect of ThinPrep preparation on human papillomavirus detection and genotyping in rectal samples by PCR

ThinPrep制备方法对PCR检测直肠样本中人乳头瘤病毒及其基因分型的影响

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作者：Tabrizi,Sepehr N,Stevens,Matthew P,van Leeuwen,Marina T,Vajdic,Claire M,Botes,Leon P,Medley,Gabriele,Hillman,Richard,Grulich,Andrew,Garland,Suzanne M
期刊：Journal of Clinical Microbiology影响因子：5.400
时间：2009起止号：2009 Jan;47(1):227-9
doi：10.1128/JCM.01518-08研究方向： 微生物学

Abstract

Specimen-to-specimen carryover during ThinPrep slide preparation was evaluated by comparing human papillomavirus genotypes detected prior and subsequent to the ThinPrep processing of 121 PreservCyt samples. Overall, 52 samples generated concordant genotypes and 38 had additional and 21 had fewer genotypes postprocessing. PreservCyt samples should be aliquoted for PCR testing prior to ThinPrep processing.

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