Abstract
Two strains, designated as Marseille-P2918(T) and Marseille-P3646(T), were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain Marseille-P2918(T) (= CSUR P2918, = DSM 103634) and Marasmitruncus massiliensis strain Marseille-P3646(T) (= CSUR P3646, = CCUG72353). Both strains were rod-shaped, anaerobic, spore forming motile bacteria. The 16S rRNA gene sequences of strains Marseille-P2918(T) (LT598554) and Marseille-P3646(T) (LT725660) shared 93.25% and 94.34% identity with Tissierella praeacuta ATCC 25539(T) and Anaerotruncus colihominis CIP 107754(T), their respective phylogenetically closest species with standing in nomenclature. Therefore, strain Marseille-P2918(T) is classified within the family Tissierellaceae and order Tissierellales whereas strain Marseille-P3646(T) is classified within the family Oscillospiraceae and order Eubacteriales. The genome of strain Marseille-P2918(T) had a size of 2.13 Mb with a GC content of 50.52% and includes six scaffolds and six contigs, and that of strain Marseille-P3646(T) was 3.76 Mbp long consisting of five contigs with a 50.04% GC content. The genomes of both strains presented a high percentage of genes encoding enzymes involved in genetic information and processing, suggesting a high growth rate and adaptability. These new taxa are extensively described and characterised in this paper, using the concept of taxono-genomic description.