Generation of two isogenic sickle cell disease induced pluripotent stem cell lines from testicular fibroblasts

从睾丸成纤维细胞中产生两种同源镰状细胞病诱导的多能干细胞系

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作者:Mitchell R Harancher, Meena Sukhwani, Carlos A Castro, Michael H Hsieh, Kyle E Orwig

Abstract

Curative bone marrow transplantation (BMT) therapies for sickle cell disease (SCD) can cause infertility. The Fertility Preservation Program (FPP) in Pittsburgh cryopreserves testicular tissues for SCD patients prior to BMT in anticipation that those tissues can be thawed in the future and matured to produce sperm. Here, we generated and validated two isogenic patient-derived induced pluripotent stem cell (iPSC) lines from testicular biopsy fibroblasts of a 12-year-old SCD patient.

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