Abstract
To investigate the interaction effects of resting times (0, 6, and 12 h) and NaHCO(3) concentrations (0, 2, 4, and 6 g/kg) on chicken myofibrillar protein (CMP), this study analyzed the changes in solubility, active sulfhydryl groups, rheological behavior, fluorescence, and gel properties of CMP solutions (60 mg/mL). The results indicated that pH significantly increased with higher NaHCO(3) concentrations and longer resting times. Consequently, solubility, active sulfhydryl groups, apparent viscosity, shear stress, G' value at 80 °C, hardness, springiness, and cohesiveness all significantly increased, while particle size, turbidity, and whiteness significantly decreased. However, these trends were not observed in samples treated with an amount of 6 g/kg NaHCO(3) and/or a resting time of 12 h. The findings suggest that treatment with 4 g/kg NaHCO(3) and a resting time of 6 h effectively reduced protein aggregation and enhanced solubility. Conversely, excessive NaHCO(3) or prolonged resting times resulted in decreased protein solubility and deteriorated textural properties.