Abstract
The optimization of volatile fatty acid (VFA) production from complex wastes under anaerobic conditions remains constrained in terms of productivity by the common use of long hydraulic retention times (HRTs, 20-30 days). Extended HRTs can limit process productivity by reducing substrate turnover and reactor throughput, while promoting further conversion of VFAs into methane and other end products. Despite its importance, the combined influence of pH and HRT on VFA yields and process optimization has not been comprehensively evaluated. This study investigates the effects of pH and short HRT on VFA production, microbial community structure, and hydrolysis and acidification efficiency in continuous stirred-tank reactors (CSTRs) fed with carbohydrate-rich feedstock (carrot residue pulp). Operating at an HRT of 11 days and an organic loading rate (OLR) of 4.4 g COD·L(-1)·d(-1) at 25 °C under pH 5.1 resulted in a VFA bioconversion efficiency of ~45% and an acidification efficiency of 84%, without compromising VFA profile or productivity compared to reactors operated at 14 days HRT and 3.3 g COD·L(-1)·d(-1). The shorter HRT and higher OLR enhanced hydrolysis efficiency (60%) and promoted greater microbial diversity, supporting robust hydrolytic activity and stable production dominated by acetic and butyric acids. These findings challenge the conventional assumption that longer retention times inherently improve process stability and demonstrate that operational conditions might improve reactor space-time yield in VFA-oriented fermentations.