Abstract
Indonesian stingless bee honey (SBH) of Geniotrigona thoracica is popular and traded at an expensive price. Brown rice syrup (RS) is frequently used as a cheap adulterant for an economically motivated adulteration (EMA) in SBH. In this study, authentic Indonesian Geniotrigona thoracica SBH of Acacia mangium (n = 100), adulterated SBH (n = 120), fake SBH (n = 100), and RS (n = 200) were prepared. In short, 2 mL of each sample was dropped directly into an innovative sample holder without any sample preparation including no dilution. Fluorescence intensity was acquired using a fluorescence spectrometer. This portable instrument is equipped with a 365 nm LED lamp as the fixed excitation source. Principal component analysis (PCA) was calculated for the smoothed spectral data. The results showed that the authentic SBH and non-SBH (adulterated SBH, fake SBH, and RS) samples could be well separated using the smoothed spectral data. The cumulative percentage variance of the first two PCs, 98.4749% and 98.4425%, was obtained for calibration and validation, respectively. The highest prediction accuracy was 99.5% and was obtained using principal component analysis-linear discriminant analysis (PCA-LDA). The best partial least square (PLS) calibration was obtained using the combined interval with R(2)(cal) = 0.898 and R(2)(val) = 0.874 for calibration and validation, respectively. In the prediction, the developed model could predict the adulteration level in the adulterated honey samples with an acceptable ratio of prediction to deviation (RPD) = 2.282, and range error ratio (RER) = 6.612.