(10494-1-AP)
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | human placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比例
- Western Blot (WB)1:5000-1:40000
- Immunoprecipitation (IP)0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
- Immunohistochemistry (IHC)1:200-1:800
- Immunofluorescence (IF)/ICC1:500-1:2000
- Flow Cytometry (FC) (INTRA)0.50 ug per 10^6 cells in a 100 µl suspension
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
文献引用 (2627)
Neuroserpin alleviates cerebral ischemia-reperfusion injury by suppressing ischemia-induced endoplasmic reticulum stress.
神经丝氨酸蛋白酶抑制剂通过抑制缺血引起的内质网应激来减轻脑缺血再灌注损伤
Chitosan alleviates symptoms of Parkinson's disease by reducing acetate levels, which decreases inflammation and promotes repair of the intestinal barrier and blood-brain barrier
壳聚糖通过降低醋酸盐水平来缓解帕金森病症状，从而减轻炎症并促进肠道屏障和血脑屏障的修复。
Hydrogen sulfide ameliorates peritoneal fibrosis: inhibition of high mobility group box-1 expression to block the activation of the transforming growth factor-beta/Smad3 pathway.
硫化氢可改善腹膜纤维化：抑制高迁移率族蛋白-1 的表达，从而阻断转化生长因子-β/Smad3 通路的激活
In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor
原位分析揭示了 TRiC 占空比和 PDCD5 作为开放状态辅因子
YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies
YTHDF2促进B细胞恶性肿瘤中的ATP合成和免疫逃逸。
Integrator loss leads to dsRNA formation that triggers the integrated stress response.
整合子丢失导致双链RNA形成，从而触发整合应激反应
Sequential simulation of regeneration-specific microenvironments using scaffolds loaded with nanoplatelet vesicles enhances bone regeneration.
利用载有纳米片囊泡的支架对再生特异性微环境进行顺序模拟，可增强骨再生
Synergistic restoration of spinal cord injury through hyaluronic acid conjugated hydrogel-polydopamine nanoparticles combined with human mesenchymal stem cell transplantation
透明质酸偶联水凝胶-聚多巴胺纳米粒子联合人间充质干细胞移植对脊髓损伤的协同修复
eEF2K regulates pain through translational control of BDNF.
eEF2K 通过 BDNF 的翻译调控来调节疼痛
PRMT1-mediated methylation regulates MLL2 stability and gene expression
PRMT1介导的甲基化调控MLL2的稳定性和基因表达
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