HIF-1 alpha Polyclonal antibody (20960-1-AP)
HIF1A; ARNT interacting protein; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Cobalt Chloride treated HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human thyroid cancer tissue, human kidney tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Cobalt Chloride treated HeLa cells, Cobalt Chloride treated HepG2 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918). .
文献引用 (255)
Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth.
核转位葡萄糖激酶作为蛋白激酶发挥作用，磷酸化TAZ并促进肿瘤生长
Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
内皮细胞 AGGF1 通过协调 TNFSF12/FN14 信号传导促进视网膜血管生成
Neuraminidase 1 Exacerbated Glycolytic Dysregulation and Cardiotoxicity by Destabilizing SIRT1 through Interactions with NRF2 and HIF1α.
神经氨酸酶 1 通过与 NRF2 和 HIF1α 相互作用，使 SIRT1 不稳定，从而加剧糖酵解失调和心脏毒性
Elevated Histone Lactylation Mediates Ferroptosis Resistance in Endometriosis Through the METTL3-Regulated HIF1A/HMOX1 Signaling Pathway.
组蛋白乳化水平升高通过 METTL3 调控的 HIF1A/HMOX1 信号通路介导子宫内膜异位症中的铁死亡抵抗
Transcriptome Landscape of Cancer-Associated Fibroblasts in Human PDAC.
人类胰腺导管腺癌相关成纤维细胞的转录组图谱
IL-21 Loading CaMnCO3 Vitality Backpacks Boost CAR-T Cell Synergistic Immunotherapy
IL-21负载CaMnCO3活力背包增强CAR-T细胞协同免疫疗法
MOTS-c attenuates lung ischemia-reperfusion injury via MYH9-Dependent nuclear translocation and transcriptional activation of antioxidant genes.
MOTS-c 通过 MYH9 依赖的核转位和抗氧化基因的转录激活来减轻肺缺血再灌注损伤
Hypoxia-induced genome-wide DNA demethylation by DNMT3A and EMT of cancer cells.
缺氧诱导的 DNMT3A 全基因组 DNA 去甲基化和癌细胞 EMT
Sequential SDF-1/CGRP-releasing smart composite hydrogel promotes osteoporotic fracture healing by targeting sensory nerve-regulated bone remodeling.
序贯释放 SDF-1/CGRP 的智能复合水凝胶通过靶向感觉神经调节的骨重塑来促进骨质疏松性骨折愈合
YY1-induced USP43 drives ferroptosis suppression by FASN stabilization and subsequent activation of SLC7A11 in ovarian cancer.
YY1 诱导的 USP43 通过 FASN 稳定和随后激活 SLC7A11 来抑制卵巢癌中的铁死亡
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