MYC tag Monoclonal antibody (60003-2-Ig)
myc; EQKLISEEDL; MYC-tag
用户验证的应用
经验证应用
| 项目 | 详情 |
|---|---|
| Positive WB detected in | Transfected HEK-293T cells, recombinant proein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
推荐稀释说明
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. Sample-dependent, Check data in validation data gallery.
详细信息
背景信息
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
文献引用 (171)
RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways.
RNF167 通过两种不同的蛋白水解途径介导 RLR 的非典型泛素化和降解
Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4.
TRIM28 的氧化还原调控通过促进 SUMO 化和抑制 OPTN 选择性自噬降解 ACSL4 来促进神经元铁死亡
A UFD1 variant encoding a microprotein modulates UFD1f and IPMK ubiquitination to play pivotal roles in anti-stress responses
UFD1 变体编码一种微蛋白，该变体调节 UFD1f 和 IPMK 的泛素化，从而在抗应激反应中发挥关键作用。
USP5-Rich Apoptotic Extracellular Vesicles Regulate Nucleus Pulposus Cells Apoptosis and DNA Damage Repair by Preventing E2F1 Proteasomal Degradation.
富含 USP5 的凋亡细胞外囊泡通过阻止 E2F1 蛋白酶体降解来调节髓核细胞凋亡和 DNA 损伤修复
EGR1 Promotes Craniofacial Bone Regeneration via Activation of ALPLâºPDGFD⺠Periosteal Stem Cells.
EGR1 通过激活 ALPLâ ºPDGFDâ º 骨膜干细胞促进颅面骨再生
DAPK3 is Essential for DBP-Induced Autophagy of Mouse Leydig Cells.
DAPK3 是 DBP 诱导小鼠睾丸间质细胞自噬所必需的
Targeting CDK2 Confers Vulnerability to Lenvatinib Via Driving Senescence in Anaplastic Thyroid Cancer.
靶向 CDK2 通过驱动间变性甲状腺癌的衰老而使其对乐伐替尼产生易感性
LIMA1 O-GlcNAcylation Promotes Hepatic Lipid Deposition through Inducing β-catenin-Regulated FASn Expression in Metabolic Dysfunction-Associated Steatotic Liver Disease.
LIMA1 O-GlcNAc糖基化通过诱导β-catenin调节的FASN表达促进代谢功能障碍相关脂肪肝疾病中的肝脏脂质沉积
BiFC and FACS-based CRISPR screening revealed that QKI promotes PABPN1 LLPS in colorectal cancer cells.
BiFC 和 FACS 筛选 CRISPR 发现 QKI 促进结直肠癌细胞中的 PABPN1 LLPS
Engineered MEVs for photoreceptor-targeted delivery of USP25 to alleviate diabetic retinopathy.
工程化MEVs用于将USP25靶向递送至光感受器，以缓解糖尿病视网膜病变
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