Abstract
Medicinal plants are promising sources of natural substances with biological functions and several drugs have been developed from traditional medicine. This study aimed to determine the chemical components of a hydromethanolic extract from Foeniculum vulgare seeds. Total phenolic, flavonoid, and flavonol contents were assessed, and gas chromatography-mass spectrometry (GC-MS) analysis was performed. To investigate the anti-inflammatory activity of F. vulgare seed hydromethanolic extract, its effects on protein denaturation, protease activity, membrane stabilization, and heat-induced hemolysis in red blood cells were evaluated in vitro. F. vulgare seed extract showed significant inhibition of protein denaturation (35.68±0.4%), protease activity (58.09±0.1%), and heat-induced hemolysis in red blood cells (9.67±0.3%) at concentrations of 200, 250, and 200 μg/mL, respectively, compared to the reference drug indomethacin (P<0.001). This remarkable anti-inflammatory activity may be attributable to the abundance of flavonoids in the F. vulgare seed extract. GC-MS confirmed the presence of linalool and fatty acids (palmitic and oleic acids), which have potential anti-inflammatory activities. Therefore, the hydromethanolic extract of F. vulgare seeds may be a valuable anti-inflammatory candidate in the years ahead.